Part:BBa_K133045

TetR-RBS-CF213-UB33-CF215-RGD-Histop

This part is regulated by tetR repressible promoter with ribosomal binding site.

It comprises of two parts of chimeric flagellin, between which we have put an urease epitope:

CF213 is the N-terminal portion of chimeric fusion protein; it contains 176 AA of the N-terminal portion of E. coli flagellin + N-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 213 AA; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.

CF215 is the C-terminal portion of chimeric fusion protein; it contains the C-terminal segment of the hypervariable region of H. pylori flagellin FlaA from 215 AA + 99 AA of C-terminal portion from E. coli flagellin; useful for inserting protein segments into the chimeric flagellin (example: CF213-multiepitope-CF215)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1417
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 759
  Illegal BamHI site found at 974
  Illegal BamHI site found at 1485
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 342
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1768